Composite

Part:BBa_K1453405

Designed by: Yangyang Li, Binhan Hao, Renhe Luo   Group: iGEM14_SJTU-BioX-Shanghai   (2014-10-17)

ssDsbA-Lgt-poxB-TAL1-His Tag

This part is used in our application test on the membrane. See more details please view iGEM14_SJTU_BioX_Shanghai.

Structure

3405.png

Figure 1 ssDsbA-Lgt-poxB-TAL1-His Tag

This part is based on the BBa_K1453402 or BBa_K1453403 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. The enzyme we used here is the pyruvate dehydrogenase (quinone) [EC:1.2.5.1] or poxB

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 862
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 483
    Illegal BsaI.rc site found at 3274
    Illegal BsaI.rc site found at 3886


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Categories
Parameters
None